Publications

Full list: Google Scholar

2024 Link to heading

Close-up photograph of red-orange desiccated soil with hexagonal cracking patterns and mineral granules, resembling evaporite deposits analogous to Martian surface conditions

Emergent ribozyme behaviors in oxychlorine brines indicate a unique niche for molecular evolution on Mars

Tanner G. Hoog, Matthew R. Pawlak, Nathaniel J. Gaut, Gloria C. Baxter, Thomas A. Bethel, Katarzyna P. Adamala & Aaron E. Engelhart

Nature Communications (2024) · Publisher

sequential gentle hydration abstract

Sequential gentle hydration increases encapsulation in model protocells

EM Gehlbach, AO Robinson, AE Engelhart, KP Adamala

Discover Life (2024) · Publisher

preprint

Alternate conformational trajectories in protein synthesis

Alternate conformational trajectories in protein synthesis

Jose L Alejo, Dylan Girodat, Michael J Hammerling, Jessica A Willi, Michael C Jewett, Aaron E Engelhart, Katarzyna P Adamala

bioRxiv (2024) · Publisher

PACRAT: Pathogen detection with aptamer-detected cascaded recombinase polymerase amplification-in vitro transcription

PACRAT: Pathogen detection with aptamer-detected cascaded recombinase polymerase amplification-in vitro transcription

Pavana Khan, Lauren Aufdembrink, Katarzyna P Adamala, Aaron Engelhart

RNA (2024) · Publisher

Controlled exchange of protein and nucleic acid signals from and between synthetic minimal cells

Controlled exchange of protein and nucleic acid signals from and between synthetic minimal cells

Joseph M Heili, Kaitlin Stokes, Nathaniel J Gaut, Christopher Deich, Jose Gomez-Garcia, Brock Cash, Matthew R Pawlak, Aaron E Engelhart, Katarzyna P Adamala

Cell Systems (2024) · doi:10.1016/j.cels.2023.12.008

preprint

Quencher-free fluorescence monitoring of G-quadruplex folding

Quencher-free fluorescence monitoring of G-Quadruplex folding

Z Parada, T Hoog, K Adamala, AE Engelhart

bioRxiv (2024) · Publisher

2023 Link to heading

preprint

High yield, low magnesium flexizyme reactions in a water-ice eutectic phase

High yield, low magnesium flexizyme reactions in a water-ice eutectic phase

Joshua Davisson, Jose Alejo, Mace Blank, Evan Kalb, Angelin Prasad, Isaac Knudson, Alanna Schepartz, Aaron E Engelhart, Katarzyna P Adamala

bioRxiv (2023) · Publisher

t7max abstract

T7Max transcription system

Christopher Deich, Brock Cash, Wakana Sato, Judee Sharon, Lauren Aufdembrink, Nathaniel J Gaut, Joseph Heili, Kaitlin Stokes, Aaron E Engelhart, Katarzyna P Adamala

J Biol Eng (2023) · doi:10.1186/s13036-023-00323-1

A gene expression control technology for cell-free systems and synthetic cells via targeted gene silencing and transfect

A gene expression control technology for cell‐free systems and synthetic cells via targeted gene silencing and transfection

Wakana Sato, Melanie Rasmussen, Nathaniel Gaut, Mahima Devarajan, Kaitlin Stokes, Christopher Deich, Aaron E Engelhart, Katarzyna P Adamala

Biotechnology and bioengineering (2023) · Publisher

New Aequorea Fluorescent Proteins for Cell-Free Bioengineering

New Aequorea Fluorescent Proteins for Cell-Free Bioengineering

C Deich, NJ Gaut, W Sato, AE Engelhart, KP Adamala

ACS Synthetic Biology (2023) · Publisher

trumpet abstract

Trumpet is an operating system for simple and robust cell-free biocomputing

Judee A. Sharon, Chelsea Dasrath, Aiden Fujiwara, Alessandro Snyder, Mace Blank, Sam O’Brien, Lauren M. Aufdembrink, Aaron E. Engelhart & Katarzyna P. Adamala

Nature communications (2023) · Publisher

Schematic diagram of three outcomes when synthetic minimal cells interact: (top row) parasite—small cell inserts DNA into a host cell; (middle row) infection—replicating genetic material transfers between cells; (bottom row) immunization by injection followed by lethal infection (Parasites, infections, and inoculation in synthetic minimal cells, ACS 2022)

Parasites, infections and inoculation in synthetic minimal cells

Brock Cash, Nathaniel J. Gaut, Christopher Deich, Laura L. Johnson, Aaron E. Engelhart, Katarzyna P. Adamala

ACS Omega (2023) · Publisher

2022 Link to heading

Expanding luciferase reporter systems for cell-free protein expression

Wakana Sato, Melanie Rasmussen, Christopher Deich, Aaron E Engelhart, Katarzyna P Adamala

Sci Rep (2022) · Publisher

DNA G-quadruplexes are uniquely stable in the presence of denaturants and monovalent cations

DNA G-quadruplexes are uniquely stable in the presence of denaturants and monovalent cations

Tanner G Hoog, Matthew R Pawlak, Benjamin F Bachan, Aaron E Engelhart

Biochemistry and Biophysics Reports (2022) · doi:10.1016/j.bbrep.2022.101238

Akaby—Cell-free protein expression system for linear templates

Akaby—Cell-free protein expression system for linear templates

Wakana Sato, Judee Sharon, Christopher Deich, Nathaniel Gaut, Brock Cash, Aaron E Engelhart, Katarzyna P Adamala

(2022) · doi:10.1101/2021.11.03.467179

Switchable DNA-Based Peroxidases Controlled by a Chaotropic Ion

Switchable DNA-Based Peroxidases Controlled by a Chaotropic Ion

Tanner G Hoog, Matthew R Pawlak, Lauren M Aufdembrink, Benjamin R Bachan, Matthew B Galles, Nicholas B Bense, Katarzyna P Adamala, Aaron E Engelhart

ChemBioChem (2022) · doi:10.1002/cbic.202200090

Programmable Fusion and Differentiation of Synthetic Minimal Cells

Programmable Fusion and Differentiation of Synthetic Minimal Cells

Nathaniel J Gaut, Jose Gomez-Garcia, Joseph M Heili, Brock Cash, Qiyuan Han, Aaron E Engelhart, Katarzyna P Adamala

ACS Synth. Biol (2022) · doi:10.1021/acssynbio.1c00519

2021 Link to heading

61575909517451

Salty Environments: The importance of evaporites and brine environments as habitats and preservers of biosignatures

(2021) · doi:10.3847/25c2cfeb.d0fffbba

2020 Link to heading

Rapid deployment of smartphone‐based augmented reality tools for field and online education in structural biology

Rapid deployment of smartphone‐based augmented reality tools for field and online education in structural biology

Tanner G Hoog, Lauren M Aufdembrink, Nathaniel J Gaut, Rou‐Jia Sung, Katarzyna P Adamala, Aaron E Engelhart

Biochemistry and Molecular Biology Education (2020) · doi:10.1002/bmb.21396

Isothermal SARS-CoV-2 diagnostics: tools for enabling distributed pandemic testing as a means of supporting safe reopeni

Isothermal SARS-CoV-2 diagnostics: tools for enabling distributed pandemic testing as a means of supporting safe reopenings

Pavana Khan, Lauren M Aufdembrink, Aaron E Engelhart

ACS Synth. Biol (2020) · doi:10.1021/acssynbio.0c00359

Highly specific, multiplexed isothermal pathogen detection with fluorescent aptamer readout

Highly specific, multiplexed isothermal pathogen detection with fluorescent aptamer readout

Lauren M Aufdembrink, Pavana Khan, Nathaniel J Gaut, Katarzyna P Adamala, Aaron E Engelhart

RNA (2020) · doi:10.1261/rna.075192.120

2019 Link to heading

Mars as a Prebiotic Chemistry Laboratory: A Martian RNA World?

T Hoog, N Gaut, MR Pawlak, BF Bachan, AE Engelhart

Mars Extant Life: What’s Next? Held 5-8 November (2019)

Methods for thermal denaturation studies of nucleic acids in complex with fluorogenic dyes

Lauren M Aufdembrink, Tanner G Hoog, Matthew R Pawlak, Benjamin F Bachan, Joseph M Heili, Aaron E Engelhart

Methods in Enzymology (2019) · doi:10.1016/bs.mie.2019.05.029

The RNA World on Mars

Tanner G. Hoog, Matthew R. Pawlak, Nathaniel J. Gaut, Gloria C. Baxter, Thomas A. Bethel, Katarzyna P. Adamala & Aaron E. Engelhart

The Astrobiology Science Conference (AbSciCon) (2019)

2018 Link to heading

Catalysis of template-directed nonenzymatic RNA copying by Iron (II)

Lin Jin, Aaron E Engelhart, Weicheng Zhang, Katarzyna Adamala, Jack W Szostak

J. Am. Chem. Soc (2018) · doi:10.1021/jacs.8b09617

Real-Time Visualization of in Vitro Transcription of a Fluorescent RNA Aptamer: An Experiment for the Upper-Division Undergraduate or First-Year Graduate Laboratory

Joseph M Heili, Jose Gomez-Garcia, Nathaniel J Gaut, Brock W Cash, Lauren M Aufdembrink, Brent A Heffron, Joshua D Shirley, Erin E Carlson, Katarzyna P Adamala, Aaron E Engelhart

Journal of Chemical Education (2018) · doi:10.1021/acs.jchemed.7b00735

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Aaron E. Engelhart

Journal of Visualized Experiments (2018) · doi:10.3791/57324

2017 Link to heading

Four structural panels (a-d) showing 3D molecular models of G-quadruplex RNA aptamer conformations: apo form with displaced dye (gray, panel a) and three ligand-bound conformations (panels b-d) with fluorescent dye molecules (blue/green) stacking on red G-tetrad layers (RNA imaging paper, Nature Chemical Biology 2017 News and Views)

RNA imaging: A tale of two G-quadruplexes

Aaron E. Engelhart

Nature Chemical Biology (2017) · doi:10.1038/nchembio.2492

2016 Link to heading

Collaboration between primitive cell membranes and soluble catalysts

Katarzyna P. Adamala, Aaron E. Engelhart (joint first author), and Jack W. Szostak

Nature Communications (2016) · doi:10.1038/ncomms11041

Primitive cell homeostasis diagram: vesicle growth dilutes inhibitory oligonucleotides and activates an encapsulated ribozyme (Engelhart, Adamala, Szostak, Nature Chemistry 2016)

A simple physical mechanism enables homeostasis in primitive cells

Aaron E. Engelhart, Katarzyna P. Adamala, and Jack W. Szostak

Nature Chemistry (2016) · doi:10.1038/nchem.2475

2015 Link to heading

Construction of a liposome dialyzer for the preparation of high-value, small-volume liposome formulations

Nature Protocols (2015) · doi:10.1038/nprot.2015.054

Generation of functional RNAs from inactive oligonucleotide complexes by non-enzymatic primer extension

JACS (2015) · doi:10.1021/ja511564d

2014 Link to heading

Structural insights into the effects of 2′-5′ linkages on the RNA duplex

PDB Entries: 4MS9 , 4MSB , 4MSR

PNAS (2014) · doi:10.1073/pnas.1317799111

Ester formation and hydrolysis during wet–dry cycles: Generation of far-from-equilibrium polymers in a model prebiotic reaction

Macromolecules (2014) · doi:10.1021/ma402256d

2013 Link to heading

Functional RNAs exhibit tolerance for non-heritable 2′–5′ versus 3′–5′ backbone heterogeneity

Nature Chemistry (2013) · doi:10.1038/nchem.1623

2012 Link to heading

Nonenzymatic ligation of DNA with a reversible step and a final linkage that can be used in PCR

ChemBioChem (2012) · doi:10.1002/cbic.201200167

2011 Link to heading

Primitive genetic polymers

Cold Spring Harbor Perspectives in Biology (2011) · doi:10.1101/cshperspect.a002196

2010 Link to heading

DNA and RNA in anhydrous media: duplex, triplex, and G-Quadruplex secondary structures in a deep eutectic solvent

Angewandte Chemie (2010) · doi:10.1002/ange.201001561

Intercalation as a means to suppress cyclization and promote polymerization of base-pairing oligonucleotides in a prebiotic world

PNAS (2010) · doi:10.1073/pnas.0914172107

2009 Link to heading

Conformational variants of duplex DNA correlated with cytosine-rich chromosomal fragile sites

J. Biol. Chem (2009) · doi:10.1074/jbc.m806866200

Evidence of strong hydrogen bonding by 8-aminoguanine

ChemComm (2009) · doi:10.1039/b818409g

Metal ion interactions with G-Quadruplex structures

RSC Book Chapter (2009) · doi:10.1039/9781847558763-00118

Sequence-Specific DNA-Metal Ion Interactions

RSC Book Chapter (2009) · doi:10.1039/9781847558763-00075

2008 Link to heading

Submicromolar, selective G-Quadruplex ligands from one pot: thermodynamic and structural studies of human telomeric DNA binding by azacyanines

ChemBioChem (2008) · doi:10.1002/cbic.200800234